Utilisation of bacteriophage display libraries to identify peptide sequences recognised by Equine herpesvirus type 1 specific equine sera
Identifieur interne : 000252 ( France/Analysis ); précédent : 000251; suivant : 000253Utilisation of bacteriophage display libraries to identify peptide sequences recognised by Equine herpesvirus type 1 specific equine sera
Auteurs : I. Birch-Machin [Royaume-Uni] ; S. Ryder [Royaume-Uni] ; L. Taylor [Royaume-Uni] ; P. Iniguez [France] ; M. Marault [France] ; L. Ceglie [Italie] ; S. Zientara [France] ; C. Cruciere [France] ; F. Cancellotti [Italie] ; G. Koptopoulos [Grèce] ; J. Mumford [Royaume-Uni] ; M. Binns [Royaume-Uni] ; N. Davis-Poynter [Royaume-Uni] ; D. Hannant [Royaume-Uni]Source :
- Journal of Virological Methods [ 0166-0934 ] ; 2000.
English descriptors
- KwdEn :
- Teeft :
- Amino, Amino acid residues, Amino acid sequences, Amino acids, Biopanning, Biopanning experiments, Clone, Consensus sequence, Conventional ponies, Crabb, Devlin, Diagnostic antigens, Diagnostic elisas, Discontinuous epitopes, Ehvc1, Ehve, Elisa, Elisa antigen, Elisa antigens, Epitope, Equine, Equine herpesvirus, Equine sera, Felici, Foal, Fuse5, Fuse5 library, Glycoprotein, Gnotobiotic, Gnotobiotic foal, Gnotobiotic foals, Herpesvirus, Homology, Microtitre plates, Misc, Negative control, Pbstg, Peptide, Peptide antigens, Peptide ehvc1, Peptide ehve, Peptide sequences, Phage, Phage clones, Phage display libraries, Phage display technology, Piii, Polyclonal sera, Protein sequences, Room temperature, Studdert, Synthetic peptides, Virol, Virological, Virological methods, Whole serum.
Abstract
Abstract: Three filamentous phage random peptide display libraries were used in biopanning experiments with purified IgG from the serum of a gnotobiotic foal infected with equine herpesvirus-1 (EHV-1) to enrich for epitopes binding to anti-EHV-1 antibodies. The sequences of the amino acids displayed were aligned with protein sequences of EHV-1, thereby identifying a number of potential antibody binding regions. Presumptive epitopes were identified within the proteins encoded by genes 7 (DNA helicase/primase complex protein), 11 (tegument protein), 16 (glycoprotein C), 41 (integral membrane protein), 70 (glycoprotein G), 71 (envelope glycoprotein gp300), and 74 (glycoprotein E). Two groups of sequences, which aligned with either glycoprotein C (gC) or glycoprotein E (gE), identified type-specific epitopes which could be used to distinguish between sera from horses infected with either EHV-1 or EHV-4 in an ELISA using either the phage displaying the peptide or synthetic peptides as antigen. The gC epitope had been previously identified as an immunogenic region by conventional monoclonal antibody screening whereas the gE antibody binding region had not been previously identified. This demonstrates that screening of phage display peptide libraries with post-infection polyclonal sera is a suitable method for identifying diagnostic antigens for viral infections such as EHV-1.
Url:
DOI: 10.1016/S0166-0934(00)00183-X
Affiliations:
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Hannant</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Animal Health Trust, Centre for Preventive Medicine, Newmarket CB8 7UU</wicri:regionArea><wicri:noRegion>Newmarket CB8 7UU</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Virological Methods</title><title level="j" type="abbrev">VIRMET</title><idno type="ISSN">0166-0934</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000">2000</date><biblScope unit="volume">88</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="89">89</biblScope><biblScope unit="page" to="104">104</biblScope></imprint><idno type="ISSN">0166-0934</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0166-0934</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>EHV-1</term><term>ELISAs</term><term>Epitope mapping</term><term>Mimotopes</term><term>Phage display libraries</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Amino</term><term>Amino acid residues</term><term>Amino acid sequences</term><term>Amino acids</term><term>Biopanning</term><term>Biopanning experiments</term><term>Clone</term><term>Consensus sequence</term><term>Conventional ponies</term><term>Crabb</term><term>Devlin</term><term>Diagnostic antigens</term><term>Diagnostic elisas</term><term>Discontinuous epitopes</term><term>Ehvc1</term><term>Ehve</term><term>Elisa</term><term>Elisa antigen</term><term>Elisa antigens</term><term>Epitope</term><term>Equine</term><term>Equine herpesvirus</term><term>Equine sera</term><term>Felici</term><term>Foal</term><term>Fuse5</term><term>Fuse5 library</term><term>Glycoprotein</term><term>Gnotobiotic</term><term>Gnotobiotic foal</term><term>Gnotobiotic foals</term><term>Herpesvirus</term><term>Homology</term><term>Microtitre plates</term><term>Misc</term><term>Negative control</term><term>Pbstg</term><term>Peptide</term><term>Peptide antigens</term><term>Peptide ehvc1</term><term>Peptide ehve</term><term>Peptide sequences</term><term>Phage</term><term>Phage clones</term><term>Phage display libraries</term><term>Phage display technology</term><term>Piii</term><term>Polyclonal sera</term><term>Protein sequences</term><term>Room temperature</term><term>Studdert</term><term>Synthetic peptides</term><term>Virol</term><term>Virological</term><term>Virological methods</term><term>Whole serum</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Three filamentous phage random peptide display libraries were used in biopanning experiments with purified IgG from the serum of a gnotobiotic foal infected with equine herpesvirus-1 (EHV-1) to enrich for epitopes binding to anti-EHV-1 antibodies. The sequences of the amino acids displayed were aligned with protein sequences of EHV-1, thereby identifying a number of potential antibody binding regions. Presumptive epitopes were identified within the proteins encoded by genes 7 (DNA helicase/primase complex protein), 11 (tegument protein), 16 (glycoprotein C), 41 (integral membrane protein), 70 (glycoprotein G), 71 (envelope glycoprotein gp300), and 74 (glycoprotein E). Two groups of sequences, which aligned with either glycoprotein C (gC) or glycoprotein E (gE), identified type-specific epitopes which could be used to distinguish between sera from horses infected with either EHV-1 or EHV-4 in an ELISA using either the phage displaying the peptide or synthetic peptides as antigen. The gC epitope had been previously identified as an immunogenic region by conventional monoclonal antibody screening whereas the gE antibody binding region had not been previously identified. This demonstrates that screening of phage display peptide libraries with post-infection polyclonal sera is a suitable method for identifying diagnostic antigens for viral infections such as EHV-1.</div></front></TEI><affiliations><list><country><li>France</li><li>Grèce</li><li>Italie</li><li>Royaume-Uni</li></country></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Birch Machin, I" sort="Birch Machin, I" uniqKey="Birch Machin I" first="I" last="Birch-Machin">I. Birch-Machin</name></noRegion><name sortKey="Binns, M" sort="Binns, M" uniqKey="Binns M" first="M" last="Binns">M. Binns</name><name sortKey="Davis Poynter, N" sort="Davis Poynter, N" uniqKey="Davis Poynter N" first="N" last="Davis-Poynter">N. Davis-Poynter</name><name sortKey="Hannant, D" sort="Hannant, D" uniqKey="Hannant D" first="D" last="Hannant">D. Hannant</name><name sortKey="Mumford, J" sort="Mumford, J" uniqKey="Mumford J" first="J" last="Mumford">J. Mumford</name><name sortKey="Ryder, S" sort="Ryder, S" uniqKey="Ryder S" first="S" last="Ryder">S. Ryder</name><name sortKey="Taylor, L" sort="Taylor, L" uniqKey="Taylor L" first="L" last="Taylor">L. Taylor</name></country><country name="France"><noRegion><name sortKey="Iniguez, P" sort="Iniguez, P" uniqKey="Iniguez P" first="P" last="Iniguez">P. Iniguez</name></noRegion><name sortKey="Cruciere, C" sort="Cruciere, C" uniqKey="Cruciere C" first="C" last="Cruciere">C. Cruciere</name><name sortKey="Marault, M" sort="Marault, M" uniqKey="Marault M" first="M" last="Marault">M. Marault</name><name sortKey="Zientara, S" sort="Zientara, S" uniqKey="Zientara S" first="S" last="Zientara">S. Zientara</name></country><country name="Italie"><noRegion><name sortKey="Ceglie, L" sort="Ceglie, L" uniqKey="Ceglie L" first="L" last="Ceglie">L. Ceglie</name></noRegion><name sortKey="Cancellotti, F" sort="Cancellotti, F" uniqKey="Cancellotti F" first="F" last="Cancellotti">F. Cancellotti</name></country><country name="Grèce"><noRegion><name sortKey="Koptopoulos, G" sort="Koptopoulos, G" uniqKey="Koptopoulos G" first="G" last="Koptopoulos">G. Koptopoulos</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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